亚洲中文字幕欧美一区,黄色三级三级黄色片,欧美黄色影视在线免费播放,久久久久精品国产亚洲av麻豆

當(dāng)前位置:首頁  >  技術(shù)文章  >  SFTSV非結(jié)構(gòu)蛋白通過與Vimentin相互作用誘導(dǎo)自噬促進病毒復(fù)制

SFTSV非結(jié)構(gòu)蛋白通過與Vimentin相互作用誘導(dǎo)自噬促進病毒復(fù)制

更新時間:2024-09-30  |  點擊率:778

20234月,天津大學(xué)生命科學(xué)學(xué)院,國家病毒性疾病預(yù)防控制研究所,病原體與生物安全國家重點實驗室,軍事醫(yī)學(xué)科學(xué)院北京微生物與流行病學(xué)研究所,天津大學(xué)環(huán)境科學(xué)與工程學(xué)院,天津市生物大分子結(jié)構(gòu)功能與應(yīng)用重點實驗室(School of Life Sciences, Tianjin University, Tianjin, China;National Institute for Viral Disease Control and Prevention, CDC, Beijing, China;State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China;School of Environmental Science and Engineering, Tianjin University, Tianjin, China;Institute of Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Tianjin, China) Yazhi Su老師研究團隊在Journal of Virology上發(fā)表論文:

The SFTSV Nonstructural Proteins Induce Autophagy to Promote Viral Replication via Interaction with Vimentin"


SFTSV非結(jié)構(gòu)蛋白通過與Vimentin相互作用誘導(dǎo)自噬促進病毒復(fù)制"


Abstract

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly identified phlebovirus associated with severe hemorrhagic fever in humans. Studies have shown that SFTSV nucleoprotein (N) induces BECN1-dependent autophagy to promote viral assembly and release. However, the function of other SFTSV proteins in regulating autophagy has not been reported. In this study, we identify SFTSV NSs, a nonstructural protein that forms viroplasm-like structures in the cytoplasm of infected cells as the virus component mediating SFTSV-induced autophagy. We found that SFTSV NSs-induced autophagy was inclusion body independent, and most phenuivirus NSs had autophagy-inducing effects. Unlike N protein-induced autophagy, SFTSV NSs was key in regulating autophagy by interacting with the host's vimentin in an inclusion body-independent manner. NSs interacted with vimentin and induced vimentin degradation through the K48-linked ubiquitin-proteasome pathway. This negatively regulating Beclin1-vimentin complex formed and promoted autophagy. Furthermore, we identified the NSs-binding domain of vimentin and found that overexpression of wild-type vimentin antagonized the induced effect of NSs on autophagy and inhibited viral replication, suggesting that vimentin is a potential antiviral target. The present study shows a novel mechanism through which SFTSV nonstructural protein activates autophagy, which provides new insights into the role of NSs in SFTSV infection and pathogenesis. IMPORTANCE Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerging tick-borne pathogen that causes multifunctional organ failure and even death in humans. As a housekeeping mechanism for cells to maintain steady state, autophagy plays a dual role in viral infection and the host's immune response. However, the relationship between SFTSV infection and autophagy has not been described in detail yet. Here, we demonstrated that SFTSV infection induced complete autophagic flux and facilitated viral proliferation. We also identified a key mechanism underlying NSs-induced autophagy, in which NSs interacted with vimentin to inhibit the formation of the Beclin1-vimentin complex and induced vimentin degradation through K48-linked ubiquitination modification. These findings may help us understand the new functions and mechanisms of NSs and may aid in the identification of new antiviral targets.

摘要:

發(fā)熱伴血小板減少綜合征病毒(SFTSV)是一種新發(fā)現(xiàn)的與人類嚴(yán)重出血熱相關(guān)的靜脈病毒。研究表明SFTSV核蛋白(N)誘導(dǎo)becn1依賴性自噬,促進病毒組裝和釋放。然而,其他SFTSV蛋白在調(diào)節(jié)自噬中的功能尚未報道。在這項研究中,研究人員鑒定了SFTSV NSs,一種在感染細(xì)胞的細(xì)胞質(zhì)中形成病毒質(zhì)樣結(jié)構(gòu)的非結(jié)構(gòu)蛋白,作為導(dǎo)SFTSV誘導(dǎo)的自噬的病毒成分。研究人員發(fā)現(xiàn)SFTSV NSs誘導(dǎo)的自噬是不依賴包涵體的,大多數(shù)phenuivirus NSs具有自噬誘導(dǎo)作用。與N蛋白誘導(dǎo)的自噬不同,SFTSV NSs通過不依賴包涵體的方式與宿主的vimentin相互作用,是調(diào)節(jié)自噬的關(guān)鍵。NSs通過k48連接的泛素-蛋白酶體途徑與波形蛋白相互作用并誘導(dǎo)波形蛋白降解。這種負(fù)調(diào)節(jié)Beclin1-vimentin復(fù)合物形成并促進自噬。此外,研究人員鑒定了vimentin的NSs結(jié)合域,發(fā)現(xiàn)野生型vimentin過表達可拮抗NSs誘導(dǎo)的自噬作用,抑制病毒復(fù)制,提示vimentin是一個潛在的抗病毒靶點。本研究揭示了SFTSV非結(jié)構(gòu)蛋白激活自噬的新機制,為NSs在SFTSV感染和發(fā)病機制中的作用提供了新的認(rèn)識。


該論文中,HeLa細(xì)胞和293T細(xì)胞的體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。



无码成人一区二区| 内射白嫩少妇超碰| 色网女人日本逼欧美| 又色又爽又黄的吃奶Av| 欧美综合区自拍亚洲综合| 少妇被黑人到高潮喷出白浆| 视频在线观看一区@99| 老司机免费福利午夜入口| 女人张开腿让男人捅个爽| 中文字幕欧美人妻在线| 2021最新热播国产一区二区| 看看美日韩操逼吧| 鸡巴操骚逼视频播放| 欧美亚洲熟妇视频在线观看| 想被操在线啊啊啊啊| 亚洲国产日韩欧美高清片| 男生操女生无马赛克免费| 三上悠亚精品一区二区久久| 精品久久av免费一区二区三区| 女生的鸡鸡色色软件| 成年女人永久看片视频 | 国产天美传媒剧免费观看| 美女被插b在线观看| 中文字幕在线资源第一页| 91成人精品国语自产拍| 日韩欧美一区二区三区在线视频| 亚洲欧洲精品无码久久久| 成人国产亚洲精品一区二| 男生狂操女生污视频| 激情五月六月婷婷俺来也| 日韩午夜经典福利| 欧美 日本 亚洲 国产| 亚洲精品自拍偷拍第一页| 亚洲欧洲日本精品| 啊啊啊操我视频大全| 一色道久久88加勒比一| 久久久久久久久黄片观看| 日韩无码av三级片| 国产精品久久久69粉嫩| 国产在线麻豆精品| 藏经阁91福利私人试看|